Thesis Flow Cytometry

We will look at the detection of disrupted PML protein in acute promyelocytic leukaemia and the ability to distinguish between cytoplasmic and nuclear NPM in acute myeloid leukaemia.The webinar will also present exciting new data demonstrating the ability to detect chromosomal abnormalities by FISH in phenotyped leukaemia cells in chronic lymphocytic leukaemia.

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FANS utilizes flow cytometry to isolate cell nuclei from CNS subtypes and microarray analysis of nuclear m RNA.

They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. In this thesis, I present a technique for profiling gene expression of specific cell types in the central nervous system (CNS), called fluorescence activated nuclei sorting (FANS).

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The imaging functionality allows the localisation of cellular antigens to be determined (i.e.

cell surface, cytoplasm, nuclear), a feature that cannot be achieved by standard flow cytometry.

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